Analysis of PSGL-1 (Human) by ELISA (CAT#: STEM-MB-1145-LGZ)

Introduction

P-Selectin Glycoprotein Ligand 1 is also called SELPLG or CD162. SELPLG encodes PSGL-1, a high-affinity antireceptor for p-selectin on myeloid cells and stimulated T lymphocytes. Thus, they play a key role in binding these cells to activated platelets or endothelial cells expressing P-selective proteins. P-selectin glycoprotein ligand 1 (PSGL-1) is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P for platelets) and is a member of the selectin family, including e-selectin (endothelial cells) and L-selectin (white blood cells). Selectins are part of a broader family of cell adhesion molecules. PSGL-1 binds to all three members of the family, but binds best to P-selectin (highest affinity).

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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