Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of PC9/PCSK9 (Human) by ELISA (CAT#: STEM-MB-1115-LGZ)

Introduction

Proprotein convertase subtilisin/kinase type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene on chromosome 1. It is the ninth member of the proprotein convertase family that activates other proteins. Like many proteins, PCSK9 is inactive when first synthesized because a portion of the peptide chain blocks its activity; proprotein convertase removes this portion to activate the enzyme. PCSK9 is ubiquitously expressed in many tissues and cell types. It binds to receptors for low-density lipoprotein particles (LDL), which normally transport 3000 to 6000 fat molecules (including cholesterol) per particle in extracellular fluid. Low-density lipoprotein receptors (LDLR) on the membranes of the liver and other cells bind and begin to uptake LDL particles from the extracellular fluid into cells, thereby reducing the concentration of LDL particles. If PCSK9 is blocked, more LDLR is recycled and present on the cell surface to clear LDL particles from the extracellular fluid. Therefore, blocking PCSK9 can reduce the concentration of LDL particles in the blood.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiac Biomarkers, Metabolic, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
Advertisement