Analysis of TFF2 (Human) by ELISA (CAT#: STEM-MB-1205-LGZ)

Introduction

Trefoil factor 2 (TFF2), is a protein encoded by the TFF2 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40 amino acid domain containing three conserved disulfide bonds. They are stable secreted proteins expressed in the gastrointestinal mucosa. Their function has not been defined, but they protect the mucosa from invasion, stabilize the mucus layer and influence the healing of the epithelium. The encoded protein inhibits gastrointestinal motility and gastric acid secretion and may function as a structural component of gastric mucus.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine

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