Analysis of CYP1B1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1714-LGZ)

Introduction

Official Full Name: cytochrome P450 family 1 subfamily B member 1<br />Also known as: CP1B; ASGD6; GLC3A; CYPIB1; P4501B1<br />This gene encodes a member of the cytochrome P450 superfamily of enzymes. Cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. The enzyme encoded by this gene is located in the endoplasmic reticulum and metabolizes carcinogenic substances such as polycyclic aromatic hydrocarbons and 17-estradiol. Mutations in this gene are associated with primary congenital glaucoma; thus, it is thought that this enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Picobirnavirus (PBV) by Real-Time PCR Method (CAT#: STEM-MB-3599-LGZ)
Analysis of SLAMF8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0654-LGZ)
Analysis of TBX19 Gene (Mutation) in Lymphatic malformation 6 by RT-qPCR (CAT#: STEM-MT-0162-LGZ)
Analysis of Treponema Hyodysenteriae by Real-Time PCR Method (CAT#: STEM-MB-3198-LGZ)
Analysis of ID3 Gene (Mutation) in Hereditary Breast Ovarian Cancer Syndrome by RT-qPCR (CAT#: STEM-MT-0088-LGZ)
Analysis of Fowl Adenovirus (FAdV-A) by Real-Time PCR Method (CAT#: STEM-MB-3791-LGZ)
Analysis of OR2T2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0547-LGZ)
Analysis of LCE3C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0411-LGZ)