Analysis of CYP1B1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1714-LGZ)

Introduction

Official Full Name: cytochrome P450 family 1 subfamily B member 1
Also known as: CP1B; ASGD6; GLC3A; CYPIB1; P4501B1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. Cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids. The enzyme encoded by this gene is located in the endoplasmic reticulum and metabolizes carcinogenic substances such as polycyclic aromatic hydrocarbons and 17-estradiol. Mutations in this gene are associated with primary congenital glaucoma; thus, it is thought that this enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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