Analysis of Denatured Proteins by Circular dichroism (CD) (CAT#: STEM-MB-0644-WXH)

Introduction

Denaturation of the proteins is a condition when the unique three-dimensional structure of a protein is exposed to changes. Due to changes in temperature, pH or other chemical activities, the hydrogen bonds present in the proteins get disturbed. Denatured proteins can exhibit a wide range of characteristics, from conformational change and loss of solubility to aggregation due to the exposure of hydrophobic groups. The loss of solubility as a result of denaturation is called coagulation. Denatured proteins lose their 3D structure and therefore cannot function.