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Study of elastin structure by Circular dichroism (CD) (CAT#: STEM-MB-0618-WXH)

Introduction

Elastin is one of the most abundant proteins in your body. It's a stretchy protein that resembles a rubber band — it can stretch out (extend) and shrink back (recoil). It's a major component of tissues in your body that require stretchiness, like your lungs, bladder, large blood vessels and some ligaments.




Principle

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Applications

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.

Procedure

1. Sample preparation
2. Measurement by CD instrument
3. Data analysis

Materials

Circular dichroism (CD) spectrophotometer
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