Analysis of DNAJB5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2045-LGZ)

Introduction

Official Full Name: DnaJ heat shock protein family (Hsp40) member B5
Also known as: Hsc40
This gene encodes a member of the DNAJ heat shock protein 40 family of co-chaperone proteins. The encoded protein contains an N-terminal DNAJ domain and a C-terminal substrate binding domain but lacks the cysteine-rich domain found in other DNAJ family members. In mice, a multi-protein complex containing this protein, thioredoxin 1, and histone deacetylase 4, serves as a master negative regulator of cardiac hypertrophy.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LOC121725126 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1598-LGZ)
Analysis of DNAJC11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1293-LGZ)
Analysis of DENND2B-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2222-LGZ)
Analysis of Gene Expression by RT-qPCR (CAT#: STEM-MB-0229-WXH)
Analysis of CALCB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2404-LGZ)
Analysis of TMEM201 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1444-LGZ)
Analysis of NELFB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1991-LGZ)
Analysis of CFHR4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0873-LGZ)