Inquiry Basket

Analysis of EHD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1801-LGZ)

Introduction

Official Full Name: EH domain containing 3
Also known as: PAST3
Predicted to enable nucleic acid binding activity. Involved in several processes, including Golgi to lysosome transport; endosomal transport; and protein homooligomerization. Acts upstream of or within protein localization to plasma membrane and regulation of cardiac muscle cell membrane potential. Located in ciliary pocket membrane and recycling endosome membrane.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LRRC52 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0436-LGZ)
Analysis of COG2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0888-LGZ)
Analysis of STK25 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1667-LGZ)
Analysis of Gallid Herpesvirus by Real-Time PCR Method (CAT#: STEM-MB-3266-LGZ)
Analysis of USH1C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2426-LGZ)
Analysis of GBA1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0144-LGZ)
Analysis of POLR1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1681-LGZ)
Analysis of ZFHX4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1866-LGZ)

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies