Analysis of EIF3H Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1910-LGZ)

Introduction

Official Full Name: eukaryotic translation initiation factor 3 subunit H<br />Also known as: EIF3S3; eIF3-p40; eIF3-gamma<br />Enables deubiquitinase activity. Contributes to translation initiation factor activity. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process and translational initiation. Located in extracellular exosome and membrane. Part of eukaryotic translation initiation factor 3 complex. Implicated in breast cancer; prostate cancer; and prostate carcinoma. Biomarker of prostate cancer.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PRAMEF20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1395-LGZ)
Analysis of PTDSS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2111-LGZ)
Analysis of STIM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2161-LGZ)
Analysis of Cryptosporidium Spp. by Real-Time PCR Method (CAT#: STEM-MB-2603-LGZ)
Analysis of Bovine Viral Diarrhea Virus 1 (BVDV-1) by Real-Time PCR Method (CAT#: STEM-MB-2744-LGZ)
Analysis of CFHR5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0872-LGZ)
Analysis of ELAPOR1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1188-LGZ)
Analysis of PMS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1501-LGZ)