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Analysis of Endoglin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0142-LGZ)

Introduction

Endoglin, also known as CD105, is a type I transmembrane glycoprotein located on the surface of the cell membrane and is one of the TGF-β receptor complexes. It is involved in the regulation of responses binding to TGF-β1, TGF-β3, Activin-A, BMP-2 and BMP-7. Endoglin is speculated to be involved in the formation of the cytoskeleton affecting cell morphology and migration. It plays a role in cardiovascular system development and vascular remodeling.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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