Analysis of Endostatin (Human) by ELISA (CAT#: STEM-MB-0781-LGZ)

Introduction

Endostatin, a 20 kDa proteolytic fragment of the C-terminal non-collagenous (NC1) domain of type XVIII collagen, is an endogenous angiogenesis inhibitor. It was originally identified as a factor produced by murine hemangioendothelioma cells that specifically inhibits endothelial cell proliferation and angiogenesis. In wound healing, it inhibits tumor growth and disrupts the maturation of blood vessels. Endostatin plays an important role in endothelial cell adhesion and cytoskeletal organization. It may also be involved in the downregulation of angiogenesis in physiological processes such as wound healing and establishment of placental circulation. The anti-angiogenic activity of endostatin has been attributed to its ability to inhibit endothelial cell proliferation and inhibit VEGF- and bFGF-induced endothelial cell migration and adhesion.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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