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Analysis of Ep-CAM (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0152-LGZ)

Introduction

Epithelial cell adhesion molecule (EpCAM), also known as CD326, is a single-channel, type I plasma membrane glycoprotein, homotype Ca2+-independent cell adhesion molecule. In addition to its role in cell-cell adhesion, in vitro and in vivo studies have shown that EpCAM plays an important role in cell signaling, proliferation, differentiation, organ formation, and maintenance. EpCAM is expressed in a variety of epithelial tissues and is also a cell surface marker of a variety of stem and progenitor cells.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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