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Analysis of EPO (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0154-LGZ)

Introduction

Erythropoietin (EPO), also known as erythropoietin, hematopoietin, or hematopoietin, is a glycoprotein cytokine secreted primarily by the kidney in response to cellular hypoxia. It stimulates the production of red blood cells (erythropoiesis) in the bone marrow. Low levels of EPO (approximately 10 mU/mL) are constantly secreted and are sufficient to compensate for normal red blood cell turnover. Common causes of cellular hypoxia that result in elevated EPO levels (up to 10,000 mU/mL) include any anemia, as well as hypoxemia due to chronic lung disease.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Rabbit Monoclonal
Detection Antibody: Goat Polyclonal
Recombinant Standards: Expressed in mouse cell line

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Cell Culture Supernatant
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