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Analysis of EPO (Rat) by ELISA (CAT#: STEM-MB-0793-LGZ)

Introduction

Erythropoietin (EPO), also known as erythropoietin, hematopoietin, or hematopoietin, is a glycoprotein cytokine secreted primarily by the kidney in response to cellular hypoxia. It stimulates the production of red blood cells (erythropoiesis) in the bone marrow. Low levels of EPO (approximately 10 mU/mL) are constantly secreted and are sufficient to compensate for normal red blood cell turnover. Common causes of cellular hypoxia that result in elevated EPO levels (up to 10,000 mU/mL) include any anemia, as well as hypoxemia due to chronic lung disease.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Hep Plasma, Serum
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