Analysis of PYY (total) (Rat) by ELISA (CAT#: STEM-MB-1151-LGZ)

Introduction

PYY shares high sequence homology and structural homology (PP-fold) with neuropeptide Y (NPY) and pancreatic polypeptide (PP), and together belong to the NPY regulatory peptide family. PYY is a linear polypeptide containing 36 amino acids, and its concentration is greatest in the terminal ileum, colon and rectum. There are mainly two forms of PYY1-36 and PYY3-36 in the circulatory system. The current research hotspot is their mechanism and role in the occurrence and treatment of obesity and diabetes. PYY can regulate appetite and body weight balance through the hypothalamic appetite regulation mechanism.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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