Analysis of ErbB2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0156-LGZ)

Introduction

erb-B2 (tyrosine kinase receptor 2, also known as NEU, CD340) is a 185kDa cell membrane receptor encoded by the proto-oncogene erbB-2, and is a member of the epidermal growth factor receptor (EGFR) family. The high expression of erb-B2 makes the cell proliferation ability stronger, the differentiation maturation and apoptosis mechanism are inhibited, and the cell malignancy is high.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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