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Analysis of ERK-1/2 (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0158-LGZ)

Introduction

Extracellular regulated protein kinases (ERK1/2) are mainly activated by phosphorylation of various growth factors, ion rays and hydrogen peroxide, and enter the nucleus to act on transcription factors such as E1k-1,c-myc,c-fos,c-jun,ATF,NF-kB and AP-1, promoting the transcription and expression of some genes, which are closely related to cell proliferation and differentiation.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence

Capture Antibody
Phospho-ERK1/2: Mouse Monoclonal
Total ERK1/2: Mouse Monoclonal

Detection Antibody
Phospho-ERK1/2: Mouse Monoclonal
Total ERK1/2: Mouse Monoclonal

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Whole Cell Lysate
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