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Analysis of F2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2459-LGZ)

Introduction

Official Full Name: coagulation factor II, thrombin<br />Also known as: PT; THPH1; RPRGL2<br />This gene encodes the prothrombin protein (also known as coagulation factor II), which undergoes multiple steps of proteolytic cleavage to form the activated serine protease thrombin. Activated thrombin converts fibrinogen to fibrin during thrombus formation and plays an important role in thrombus formation and hemostasis by stimulating platelet aggregation and activating other coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis, and maintains vascular integrity during development and after birth. Peptides derived from the C-terminus of this protein have antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysthrombinemia. The rapid increase in cytokine levels following coronavirus infection can dysregulate the coagulation cascade, producing thrombus, impaired blood supply, and organ failure.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements