Analysis of Factor VII (Human) by ELISA (CAT#: STEM-MB-0803-LGZ)

Introduction

Factor VII (proconvertin) is one of the proteins in the coagulation cascade that causes blood to coagulate. It is an enzyme of the serine protease class. Once bound to tissue factor released from damaged tissue, it is converted to factor VIIa (or factor VIIa, activated factor VII), which in turn activates factors IX and X. The action of this factor is blocked by tissue factor pathway inhibitor (TFPI), which is released almost immediately after blood clotting begins.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Plasma, Saliva, Serum

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