Analysis of FGF (basic) (Human) by ELISA (CAT#: STEM-MB-0810-LGZ)

Introduction

Fibroblast growth factor (FGF) has several isomers, and the main one that plays a role in arteriosclerosis is basic fibroblast growth factor (bFGF). bFGF can be secreted by endothelial cells, smooth muscle cells, and macrophages. Its function is to promote the migration of endothelial cells and the proliferation of smooth muscle cells, but cannot make smooth muscle cells migrate. It can promote the formation of new blood vessels and repair damaged endothelial cells. FGF is considered a lesion-promoting factor, but it also has a positive side from a repair perspective.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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