Analysis of FGF20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1919-LGZ)

Introduction

Official Full Name: fibroblast growth factor 20
Also known as: RHDA2; FGF-20
The protein encoded by this gene is a member of the fibroblast growth factor family. Fibroblast growth factors have a wide range of mitotic and cell survival activities, and participate in various biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The gene product is a secreted neurotrophic factor but lacks a typical signal peptide. It is expressed in the normal brain, especially the cerebellum, and may regulate central nervous system development and function. Homodimerization of this protein was shown to modulate its receptor binding activity and concentration gradient in the extracellular matrix. Genetic variation in this gene has been linked to susceptibility to Parkinson's disease.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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