Analysis of Flu B/Phuket HA (Human) by ELISA (CAT#: STEM-MB-0825-LGZ)

Introduction

There are four types of influenza viruses: A, B, C, and D. In the United States, influenza A and B viruses cause seasonal epidemics of illness (known as flu season) in humans almost every winter. Influenza A viruses are the only influenza viruses known to cause influenza pandemics (i.e. worldwide epidemics of influenza disease). A pandemic can occur when a new and different influenza A virus emerges to infect humans with the ability to spread efficiently in a population against which people have little or no immunity. Influenza C virus infection usually causes mild illness and is not thought to cause human epidemics. Influenza D viruses primarily affect cattle and are not known to infect or cause illness in humans.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Respiratory

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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