Analysis of FMO4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0999-LGZ)

Introduction

Official Full Name: flavin containing dimethylaniline monoxygenase 4<br />Also known as: FMO2<br />Metabolic n-oxidation of dietary aminotrimethylamine (TMA) is mediated by flavin-containing monooxygenases and influenced by inherited FMO3 polymorphisms in humans. This results in decreased TMA n oxidation capacity in a small proportion of the population and leads to fish odor syndrome (trimethylaminuria). The three forms of the enzyme are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are Nadph-dependent diaphorases that catalyze the oxidation of nucleophilic soft heteroatom centers in drugs, pesticides, and xenobiotics.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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