Analysis of PD-L1 by RT-qPCR (CAT#: STEM-MT-0050-LGZ)

Introduction

Multiple biomarkers are being evaluated to guide the use of immune checkpoint inhibitors in non-small cell lung cancer (NSCLC), including staining of programmed death-ligand 1 (PD-L1) tumor cells. We can accurately quantify PD-L1 status and identify multiple mutations by using a single bronchoscopic specimen.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Non–Small-Cell Lung Cancer (NSCLC)

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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