Analysis of Fowl Pox Virus (FPV) by Real-Time PCR Method (CAT#: STEM-MB-3151-LGZ)

Introduction

The Fowl pox virus (FPV) is a DNA virus that belongs to the family Poxviridae, subfamily Chordopoxvirinae, genus Avipoxvirus, in addition to pigeon pox virus (PPV) and turkey pox virus (TPV). The Fowl pox virus affects poultry and influences on egg production.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.