Analysis of Fructus Tsaoko by Real-Time PCR Method (CAT#: STEM-MB-3989-LGZ)

Introduction

Fructus tsaoko (Amomum tsaoko), also named tsaoko, is a perennial shade tolerance herb of Zingiberaceae family, Amomum genus, and grows in high altitude areas that are cool in summer and warm in winter, in enriched soil and under forest shading ranging from 50% to 60%.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.