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Analysis of G-CSF (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0202-LGZ)

Introduction

Granulocyte colony stimulating factor (G-CSF) is a glycoprotein containing 174 amino acids with a molecular weight of about 20,000. G-CSF mainly acts on the proliferation, differentiation and activation of lineage hematopoietic cells. Clinically, G-CSF is mainly used for the prevention and treatment of leukopenia caused by radiotherapy or chemotherapy, the treatment of myelopoietic dysfunction and myelodysplastic syndrome, the prevention of potential infectious complications of leukopenia, and to accelerate the recovery of neutropenia caused by infection.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation, Cytokines & Chemokines

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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