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Analysis of GAS5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0992-LGZ)

Introduction

Official Full Name: growth arrest specific 5
Also known as: SNHG2; NCRNA00030
This gene produces a spliced long non-coding RNA and is a member of the 5' oligopyrimidine class of genes. It is a small nucleolar RNA host gene with multiple C/D box snoRNA genes in its intron. Part of the secondary RNA structure encoding the transcript mimics the glucocorticoid response element (GRE), meaning it can bind to the DNA-binding domain of the glucocorticoid receptor (nuclear receptor subfamily 3, group C, member 1). This action prevents the glucocorticoid receptor from being activated, thereby preventing it from regulating the transcription of target genes. This transcript is also thought to regulate the transcriptional activity of other receptors, such as the androgen, progesterone, and mineralocorticoid receptors, which bind to their GRE-mimicking regions. Multiple functions are associated with this transcript, including cell growth arrest and apoptosis. It is also considered a potential tumor suppressor and its downregulation has been linked to cancers in a variety of different tissues.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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