Analysis of GBX2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1775-LGZ)

Introduction

Official Full Name: gastrulation brain homeobox 2<br />Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of nervous system development and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including branching involved in blood vessel morphogenesis; nervous system development; and neural crest cell migration. Predicted to be part of chromatin. Predicted to be active in nucleus.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Human Papillomavirus 11 (HPV-11) by Real-Time PCR Method (CAT#: STEM-MB-3345-LGZ)
Analysis of Duck Hepatitis Virus 3(DHV-3) by Real-Time PCR Method (CAT#: STEM-MB-2572-LGZ)
Analysis of CCL27 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2049-LGZ)
Analysis of OR6B3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1655-LGZ)
Analysis of TMEM88B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1232-LGZ)
Analysis of PASK Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1653-LGZ)
Analysis of ACTA2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2082-LGZ)
Analysis of AIM2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0763-LGZ)