Analysis of GDNF/ATF1/ATF2 by ELISA (CAT#: STEM-MB-1539-LGZ)

Introduction

Glial cell derived neurotrophic factor (GDNF) is a neurotrophic factor that promotes the survival of different neuronal subsets at different stages of development in both the central and peripheral nervous systems. The functional human GDNF ligand is a disulfide bonded homologous dimer consisting of two 15kDa polypeptide chains. GDNF is produced by sersupport cells, type 1 astrocytes, Schwann cells, neurons, pineal cells, and skeletal muscle cells. It is a secretory protein that promotes the survival of motor neurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. The survival efficiency of GDNF on spinal motor neurons was 100 times higher than that of neurotrophic protein. In mouse models of Parkinson's disease, GDNF has been found to improve conditions such as slow movement, stiffness and postural instability.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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