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Analysis of CEACAM-5 (Human) by ELISA (CAT#: STEM-MB-0738-LGZ)

Introduction

Carcinoembryonic antigen (CEA), also known as CD66e and CEACAM5, is a glycoprotein with a molecular weight of 180-200kD involved in cell adhesion. CEA is usually produced in the fetal digestive system, but because it is suppressed after birth, the content of CEA in the colon and blood of normal adults is usually very low. However, elevated serum levels of CEA can be caused by various types of cancer including colorectal, lung, and breast cancers. Therefore, CEA is widely used as a broad-spectrum tumor marker. CEA is not specific for any kind of cancer, but the concentration of CEA in blood can predict the therapeutic effect, progress and prognosis of cancer.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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