Analysis of α-GST (Human) by ELISA (CAT#: STEM-MB-1272-LGZ)

Introduction

Glutathione S-transferase A1 is an enzyme encoded by the GSTA1 gene. The cell membrane and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function by conjugating glutathione to detoxify electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress. It is well known that the genes encoding these enzymes are highly polymorphic. These genetic variations can alter a person's susceptibility to carcinogens and toxins and affect the toxicity and efficacy of some drugs. Currently, eight distinct classes of soluble cytoplasmic mammalian glutathione S-transferases have been identified: α, kappa, mu, omega, pi, sigma, theta, and zeta.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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