Analysis of H3C15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1055-LGZ)

Introduction

Official Full Name: H3 clustered histone 15
Also known as: H3/n; H3/o; H3C13; H3C14; HIST2H3A
Histones are fundamental nuclear proteins responsible for the structure of eukaryotic chromosome fibers in the nucleosome. The structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of each pair of four core histones (H2A, H2B, H3, and H4). By linking DNA interactions between histone H1 and nucleosomes, chromatin fibers are further compacted to form higher-order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts of this gene lack poly-A tails; instead, they contain a palindromic termination element. This gene is present in a histone cluster on chromosome 1. This gene is one of four duplicated histone genes in the cluster; this record represents the centromeric copy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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