Analysis of H6PD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1330-LGZ)

Introduction

Official Full Name: hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase
Also known as: GDH; G6PDH; H6PDH; CORTRD1
There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of CENPF Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0848-LGZ)
Analysis of Singapore Grouper Iridovirus (SGIV) by Real-Time PCR Method (CAT#: STEM-MB-3590-LGZ)
Analysis of DUSP28 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1568-LGZ)
Analysis of SMIM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1424-LGZ)
Analysis of Boheliridovirus (BIV) by Real-Time PCR Method (CAT#: STEM-MB-3558-LGZ)
Detection of Common MPL Mutations in Myeloproliferative Neoplasms (MPNS) by RT-qPCR (CAT#: STEM-MT-0020-LGZ)
Analysis of FAM245A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2072-LGZ)
Analysis of Norwalk Viruses G3 (NV-3) by Real-Time PCR Method (CAT#: STEM-MB-3235-LGZ)