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Analysis of HAAO Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1737-LGZ)

Introduction

Official Full Name: 3-hydroxyanthranilate 3,4-dioxygenase
Also known as: HAO; 3-HAO; VCRL1; h3HAO
3-Hydroxybenzoate 3,4-dioxygenase is a monomeric cytosolic protein that belongs to the family of intramolecular dioxygenases containing non-heme ferrous. It is widely distributed in peripheral organs such as liver and kidney, and also exists in a small amount in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxybenzoic acid. QUIN is an excitotoxin whose toxicity is mediated through its ability to activate glutamate N-methyl-D-aspartate receptors. Elevated levels of QUIN in the brain may be involved in the pathogenesis of neurological and inflammatory diseases. HAAO has been implicated to play a role in diseases associated with altered tissue levels of QUIN.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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