Analysis of Haplosporidium Costale by Real-Time PCR Method (CAT#: STEM-MB-3553-LGZ)

Introduction

The protist Haplosporidium costale belonging to the phylum Haplosporidia mainly infects the eastern oyster Crassostrea virginica. This parasite was first reported to cause high mortalities in one and two years-old C. virginica along the Atlantic coast of Virginia in the 1960s.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.