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Analysis of BIN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1698-LGZ)

Introduction

Official Full Name: bridging integrator 1<br />Also known as: CNM2; AMPH2; AMPHL; SH3P9<br />This gene encodes several isoforms of the nucleocytoplasmic adapter protein, one of which was originally identified as a myc-interacting protein with features of a tumor suppressor. The isoform expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynein, synapsin, endophilin, and clathrin. The isoform expressed in muscle and the ubiquitously expressed isoform localized to the cytoplasm and nucleus and activated caspase-independent apoptotic processes. Mouse studies have shown that the gene plays an important role in heart muscle development. Alternative splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements