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Analysis of Hemopexin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0251-LGZ)

Introduction

Hemopexin (Hpx) is a single-chain polypeptide, an acute phase response protein synthesized by the liver. It has a specific binding ability to free heme. When extensive hemolysis occurs, plasma haptoglobin is depleted, and free hemoglobin in the circulation can be degraded into globin and heme. Heme is insoluble in water and can be combined with Hpx to form a complex and transported to the liver.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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