Analysis of Human T-cell Lymphoma Virus Provirus (HTLV-1) by Real-Time PCR Method (CAT#: STEM-MB-3271-LGZ)

Introduction

The human T-lymphotropic virus type 1 is also known by the acronym HTLV-1, or as human T-cell leukaemia virus type 1. The virus can cause a type of cancer called adult T-cell leukaemia/lymphoma (ATL). HTLV-1 is transmitted primarily through infected bodily fluids including blood, breast milk and semen.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.