Analysis of IGFBP-6 by ELISA (CAT#: STEM-MB-1546-LGZ)

Introduction

The insulin-like growth factor (IGF) binding protein superfamily includes six high-affinity IGf-binding proteins (IGFBP) and at least four low-affinity binding proteins known as igFBP-associated proteins (IGFBP-RP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues. IGFBPs help extend the half-life of IGFs in all tissues, including the prostate. In humans, insulin-like growth factor binding protein 6(IGFBP-6) is a protein encoded by the IGFBP6 gene. IGFBP-6 binds to IGF-II with a higher affinity than IGF-I, and it is a specific inhibitor of IGF-II. IGFBP-6 has been reported to play a role in cell proliferation, survival, differentiation, and migration independent of IGF. IGFBP-6 is unique among IGFBPs due to its clear IGF-II binding preference.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabonomics

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

Other recommended products

Analysis of MCP-3 (Human) by ELISA (CAT#: STEM-MB-1038-LGZ)
Analysis of Mouse DAO(Diamine Oxidase) by ELISA (CAT#: STEM-MB-1937-LGZ)
Analysis of Human GRP78 (Glucose Regulated Protein 78) by ELISA (CAT#: STEM-MB-1908-LGZ)
Analysis of Histone H3 (Human, Mouse, Rat) by ELISA (CAT#: STEM-MB-0893-LGZ)
Analysis of Rat ADH(Antidiuretic Hormone) by ELISA (CAT#: STEM-MB-1657-LGZ)
Analysis of Rat FETUA(Fetuin A) by ELISA (CAT#: STEM-MB-2128-LGZ)
Analysis of Rat CTNNβ1(Catenin, Beta 1) by ELISA (CAT#: STEM-MB-2019-LGZ)
Analysis of Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) by ELISA (CAT#: STEM-MB-2052-LGZ)