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Analysis of IL-12/IL-23p40 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0295-LGZ)

Introduction

Interleukin-12 (IL-12) is a cytokine with a wide range of biological activities, mainly produced by activated inflammatory cells. Interleukin-23 (IL-23), composed of the 40kD subunits of p19 and IL-12, is secreted by activated DC and can promote the proliferation of CD45RO+ memory T cells and produce interferon-γ. IL-23, a new member of the IL-12 family, shares a common P40 subunit with IL-12




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Expressed in Insect cell line

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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