Analysis of INSC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2394-LGZ)

Introduction

Official Full Name: INSC spindle orientation adaptor protein<br />In Drosophila, neuroblasts divide asymmetrically into another neuroblast at the apical side and a smaller ganglion mother cell on the basal side. Cell polarization is precisely regulated by 2 apically localized multiprotein signaling complexes that are tethered by Inscuteable, which regulates their apical localization.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of TMEM240 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1443-LGZ)
Analysis of H2AC20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1025-LGZ)
Analysis of RIF1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1476-LGZ)
Analysis of Ehrlichia Chaffeensis by Real-Time PCR Method (CAT#: STEM-MB-3353-LGZ)
Analysis of LCE1D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1120-LGZ)
Analysis of Human Parainfluenza Virus 4A (HPIV-4A) by Real-Time PCR Method (CAT#: STEM-MB-3859-LGZ)
Analysis of UBQLNL Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2169-LGZ)
Analysis of Clostridium Botulinum TypeA by Real-Time PCR Method (CAT#: STEM-MB-2648-LGZ)