Analysis of Insulin (Human, Non-human primate, Canine) by ELISA (CAT#: STEM-MB-1005-LGZ)

Introduction

Insulin is a protein hormone secreted by the islet β cells in the pancreas when stimulated by endogenous or exogenous substances such as glucose, lactose, ribose, arginine, and glucagon. Insulin is the only hormone in the body that lowers blood sugar, and at the same time promotes the synthesis of glycogen, fat, and protein. Insulin is an important polypeptide hormone that regulates carbohydrate metabolism. Abnormal level of insulin is associated with diabetes mellitus characterized by chronic hyperglycemia. Therefore, reliable quantification of insulin is important for clinical purpose such as diabetes diagnosis and treatment.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Plasma, Serum

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