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Analysis of Interaction between Tartrazine and Serum Albumins by UV-Vis Spectroscopy (CAT#: STEM-MB-0932-WXH)

Introduction

Tartrazine is the second most widely used food coloring agent. It is added to a broad range of foods such as soft drinks, chips, pudding, honey, pickles, gum, mustard, gelatin, and baked goods. Tartrazine is a bright yellow azo dye that is more stable and a cheaper alternative to natural food dyes.
Serum albumins (SAs), the most abundant proteins in blood plasma, play important roles in the transport and deposition of a variety of endogenous and exogenous substances including fatty acids, amino acids, metals, and pharmaceuticals in blood. The binding of toxins to serum albumins may have physiological importance, because they controls their free, active concentrations and affect the duration and intensity of their effects.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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