Analysis of Interactions of tetracyclines with ovalbumin by UV-Vis Spectroscopy (CAT#: STEM-MB-0991-WXH)

Introduction

Tetracyclines (tetracycline, doxycycline, minocycline, tigecycline) are a class of medication used to manage and treat various bacterial infections. Tetracyclines classify as protein synthesis inhibitor antibiotics and are considered to be broad-spectrum.
Ovalbumin (OVA) is a key reference protein for vaccination experiments. Ovalbumin, the major protein constituent of chicken egg whites, is a glycoprotein that is sufficiently large and complex to be mildly immunogenic. Consequently, it is widely used as an antigen for immunization research.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer

Other recommended products

Analysis of interactions between Schiff base and DNA/protein by UV-Vis Spectroscopy (CAT#: STEM-MB-0985-WXH)
Characterization of Green Fluorescent Protein by UV-Vis Spectroscopy (CAT#: STEM-MB-0945-WXH)
Analysis of interactions of bioactive vanadium species with bovine serum albumin by UV-Vis Spectroscopy (CAT#: STEM-MB-0997-WXH)
Analysis of Interaction between Tartrazine and Serum Albumins by UV-Vis Spectroscopy (CAT#: STEM-MB-0932-WXH)
Lysozyme monolayer-protected gold nanoparticles (AuNPs) by UV-Vis Spectroscopy (CAT#: STEM-MB-0952-WXH)
Characterization of chitosan/ZnO nanoparticle composite membranes by UV-Vis Spectroscopy (CAT#: STEM-MB-0923-WXH)
Monitering the fermentation of Escherichia coli by UV-Vis Spectroscopy (CAT#: STEM-MB-0878-WXH)
Analysis of organic pollutant-protein interactions by UV-Vis Spectroscopy (CAT#: STEM-MB-0988-WXH)