Analysis of KLHL12 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1105-LGZ)

Introduction

Official Full Name: kelch like family member 12<br />Also known as: DKIR; C3IP1<br />This gene encodes a member of the KLHL (kelch-like) protein family. This protein has been identified as an autoantigen for the autoimmune disease Sjogren's syndrome and as a potential biomarker for primary biliary cirrhosis. This protein may serve as a substrate linker for the Cullin-3 ubiquitin ligase complex, promoting substrate-specific ubiquitination. Ubiquitination of this complex has been shown to regulate Wnt signaling as well as COPII vesicle coat size. A pseudogene was found on chromosome 22. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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