Analysis of LCE1A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1093-LGZ)

Introduction

Official Full Name: late cornified envelope 1A<br />Also known as: LEP1<br />LCE1A belongs to the late cornified envelope (LCE) gene cluster within the epidermal differentiation complex (EDC) on chromosome 1. The LCE cluster contains multiple conserved genes that encode stratum corneum proteins, and these genes are expressed relatively late during fetal assembly of the skin cornified envelope.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of BIRC6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1795-LGZ)
Analysis of Norwalk Viruses G1 (NV-1) by Real-Time PCR Method (CAT#: STEM-MB-3233-LGZ)
Analysis of KIAA1614 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1075-LGZ)
Analysis of Adenovirus (AV) - Type E by Real-Time PCR Method (CAT#: STEM-MB-3451-LGZ)
Analysis of Cytoplasmic Polyhedrosis Virus (CPV) by Real-Time PCR Method (CAT#: STEM-MB-2592-LGZ)
Analysis of MTRNR2L8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2306-LGZ)
Vectorette PCR (CAT#: STEM-MB-0222-WXH)
Analysis of CTTNBP2NL Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1167-LGZ)