Analysis of QSOX1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0602-LGZ)

Introduction

Official Full Name: quiescin sulfhydryl oxidase 1<br />Also known as: Q6; QSCN6<br />The gene encodes a protein containing domains of thioredoxin and ERV1, members of two long-standing gene families. This gene is induced when fibroblasts begin to exit the proliferative cycle and enter a quiescent state, suggesting that this gene plays an important role in growth regulation. Two transcript variants of this gene have been found encoding two different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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