Analysis of LCN10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1994-LGZ)

Introduction

Official Full Name: lipocalin 10
Members of the lipocalin family, such as LCN10, have a common structure consisting of an 8-stranded antiparallel beta-barrel that forms a cup-shaped ligand-binding pocket or calyx. Lipocalins generally bind small hydrophobic ligands and transport them to specific cells.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of INS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2264-LGZ)
Analysis of Fructus Momordicae by Real-Time PCR Method (CAT#: STEM-MB-3961-LGZ)
Analysis of Histoplasma Capsulatum Var. Duboisii by Real-Time PCR Method (CAT#: STEM-MB-3833-LGZ)
Analysis of Corynebacterium Renale by Real-Time PCR Method (CAT#: STEM-MB-3059-LGZ)
Analysis of OR6A2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2375-LGZ)
Analysis of OR56B1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2380-LGZ)
Asymmetric PCR (CAT#: STEM-MB-0192-WXH)
Analysis of Mycobacterium Microti by Real-Time PCR Method (CAT#: STEM-MB-3565-LGZ)