Analysis of LIF (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0386-LGZ)

Introduction

Leukemia inhibitory factor (LIF) has the ability to induce terminal differentiation of leukemia cells. Its activities include inducing hematopoietic differentiation of normal and myeloid leukemia cells, inducing neuronal cell differentiation, and stimulating acute phase protein synthesis in hepatocytes.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Bone Disorders, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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